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首页> 外文期刊>Journal of Biotechnology >Effect of pH and soybean cultivars on the quantitative analyses of soybean rhizobia populations
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Effect of pH and soybean cultivars on the quantitative analyses of soybean rhizobia populations

机译：pH和大豆品种对大豆根瘤菌种群数量分析的影响

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摘要

Quantitative analyses of fast- and slow-growing soybean rhizobia populations in soils of four different provinces of China (Hubei, Shan Dong, Henan, and Xinjiang) have been carried out using the most probable number technique (MPN). All soils contained fast- (FSR) and slow-growing (SSR) soybean rhizobia. Asiatic and American soybean cultivars grown at acid, neutral and alkaline pH were used as trapping hosts for FSR and SSR strains. The estimated total indigenous soybean-rhizobia populations of the Xinjiang and Shan Dong soil samples greatly varied with the different soybean cultivars used. The soybean cultivar and the pH at which plants were grown also showed clear effects on the FSR/SSR rations isolated from nodules. Results of competition experiments between FSR and SSR strains supported the importance of the soybean cultivar and the pH on the outcome of competition for nodulation between FSR and SSR strains. In general, nodule occupancy by FSRs significantly increased at alkaline pH. Bacterial isolates from soybean cultivar Jing Dou 19 inoculated with Xinjiang soil nodulate cultivars Heinong 33 and Williams very poorly. Plasmid and lipopolysaccharide (LPS) profiles and PCR-RAPD analyses showed that cultivar Jing Dou 19 had trapped a diversity of FSR strains. Most of the isolates from soybean cultivar Heinong 33 inoculated with Xinjiang soil were able to nodulate Heinong 33 and Williams showed very similar, or identical, plasmid, LPS and PCR-RAPD profiles. All the strains isolated from Xinjiang province, regardless of the soybean cultivar used for trapping, showed similar nodulation factor (LCO) profiles as judged by thin layer chromatographic analyses. These results indicate that the existence of soybean rhizobia sub-populations showing marked cultivar specificity, can affect the estimation of total soybean rhizobia populations indigenous to the soil, and can also affect the diversity of soybean rhizobial strains isolated from soybean nodules.
机译：使用最可能数技术（MPN）对中国四个不同省（湖北，山东，河南和新疆）土壤中速生和慢生的大豆根瘤菌种群进行了定量分析。所有土壤均含有速生（FSR）和慢生（SSR）大豆根瘤菌。在酸性，中性和碱性pH下生长的亚洲和美国大豆品种被用作FSR和SSR菌株的诱集宿主。新疆和山东土壤样本中估计的本地大豆-根瘤菌总种群随所用大豆品种的不同而有很大差异。大豆品种和植物生长的pH值对从结节分离的FSR / SSR配比也显示出明显的影响。 FSR和SSR菌株竞争试验的结果支持了大豆品种的重要性和pH值对FSR和SSR菌株结瘤竞争结果的影响。通常，在碱性pH值下，FSR的根瘤占有率显着增加。用新疆土壤结核病品种Heinong 33和Williams接种的大豆品种京豆19的细菌分离物非常差。质粒和脂多糖（LPS）谱以及PCR-RAPD分析表明，景豆19品种已捕获了多种FSR菌株。用新疆土壤接种的大豆品种Heinong 33的大多数分离株均能结瘤Heinong 33，Williams显示出非常相似或相同的质粒，LPS和PCR-RAPD图谱。通过薄层色谱分析可知，从新疆省分离出的所有菌株，无论用于诱集的大豆品种如何，均显示出相似的结瘤因子（LCO）谱。这些结果表明，大豆根瘤菌亚群的存在具有明显的栽培品种特异性，可以影响土壤中大豆根瘤菌总种群的估计，还可以影响从大豆根瘤中分离出来的大豆根瘤菌菌株的多样性。

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