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首页> 外文期刊>Journal of Arboriculture >Identification of drought-tolerant woody perennials using chlorophyll fluorescence
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Identification of drought-tolerant woody perennials using chlorophyll fluorescence

机译：利用叶绿素荧光鉴定耐旱木本多年生植物

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The purpose of this research was to determine whether chlorophyll fluorescence values obtained from excised leaves of woody plants subjected to dehydration in vitro provided a measurable indicator of whole-plant performance following drought in situand to gain a greater understanding of alterations in leaf photosynthctic properties between species. Based on reductions in photochemical efficiency, as measured by chlorophyll fluorescence, of detached leaves of 30 woody plants in vitro following 24 hours of dehydration, plants were ranked in order of tolerance. Five species identified as drought tolerant, intermediate, and sensitive were subjected to 70 days of drought under glasshouse conditions. Based on mortality rates at day 70. drought tolerance followed the same order as that obtained in vitro. In addition, reductions in chlorophyll fluorescence parameters and photosynthetic rates of whole plants mirrored tolerance ranking in vitro (i.e., rates declined most rapidly in species identified as dehydration sensitive and least in species identified as drought tolerant). Alteration to leaf chlorophyll fluorescence parameters in the test species highlighted a number of previously unrcported effects on the leaf photosynthctic apparatus in response to drought. Results strongly indicate that screening of detached leaf material in vitro vising chlorophyll fluorescence can provide a means of gauging the drought tolerance of plants with limited whole-plant experimentation.
机译：这项研究的目的是确定从木本植物的离体叶片中进行体外脱水获得的叶绿素荧光值是否可作为原位干旱后全植物性能的可衡量指标，从而更好地了解物种之间叶片光合特性的变化。 。脱水24小时后，根据30种木本植物离体叶片的光化学效率降低（通过叶绿素荧光测量），对植物进行了耐性排序。在温室条件下，五个被确定为耐旱，中度和敏感干旱的物种遭受了70天的干旱。基于第70天的死亡率，干旱耐受性与体外获得的顺序相同。另外，整个植物的叶绿素荧光参数的降低和光合速率的降低反映了体外的耐性等级（即，在被确定为对脱水敏感的物种中，速率下降最快，而在被鉴定为对干旱具有耐受性的物种上下降最快）。在测试物种中，叶绿素荧光参数的改变突显了干旱之前对叶片光合装置产生的许多先前无法报告的作用。结果强烈表明，通过叶绿素荧光体外筛选离体叶片材料可以提供一种通过有限的全植物试验来测量植物的耐旱性的手段。

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