摘要:[目的]研究直投式乳酸菌剂对肥胖大鼠肝脏脂肪蓄积的影响.[方法]60只雄性,SPF级SD大鼠适应性喂养3d后,按照体重随机分为5组,分别为对照组,肥胖模型组,低、中、高剂量直投式乳酸菌剂组,对照组和模型组分别喂饲基础饲料和高脂饲料,并灌胃蒸馏水,低、中、高剂量组喂饲高脂饲料并分别灌胃1.05× 109cfu/kg/d,2.10×109cfu/kg/d,4.20× 109cfu/kg/d的直投式乳酸菌悬液.每天记录各组大鼠的摄食情况,每周称体重,15周后,处死所有大鼠,取肝脏称重,计算肝体比;HE染色观察肝脏组织病理变化;并测定肝脏组织中TG、TC的含量.[结果]肥胖模型组大鼠平均每日能量摄入、体重、肝体比、肝脏中TG和TC含量均显著高于对照组(P<0.05,或P<0.01),高脂饲料成功造成了大鼠肥胖模型.与模型组相比,高剂量菌剂组大鼠平均膳食及能量摄入量均显著降低(P<0.01),而各实验组大鼠体重较模型组均无统计学差异;高剂量组大鼠的肝体比及三个实验组的肝脏TG显著低于肥胖组大鼠(P<0.01).直投式乳酸菌实验组肝脏细胞脂肪变性以及炎性细胞的浸润减少.[结论]直投式乳酸菌剂能显著减少肥胖大鼠肝脏脂肪蓄积,具有一定抗脂肪肝作用.%[Objective] the study was designed to investigate the effects of direct vat set lactobacillus preparation (DLP) on the fat accumulation in liver of obesity rats. [Methods] After 3 days adaptive feeding, a total of 60 SD rats were randomly divided into five groups, control group fed conventional diet, obesity model group fed high-fat diet, the low-, the middle-, and the high-dose experimental group fed high-fat diet and infused with the solution of DLP at levels of 1.05*10'cfu/kg/d, 2.10*109cfu/kg/d,4.20*10'cfu/kg/d, respectively. The food intake was recorded every day, and body weight was determined weekly. At 15 weeks, all rats were killed, and the liver was removed and liver/body weight ratio (LBR) was calculated; then the liver was sectioned for observing the change of liver pathology. The contents of TG and TC were also measured using commercial kits. [Results] the average energy intake, body weight, LBR, TG and TC in liver of obesity model group were significantly higher than that in controls (P0.05). The LBR in high-dose group and TG in three experimental groups reduced remarkably in comparison with the model group (P<0.01). The steatosis and inflammatory infiltration in liver of experimental groups were improved to some degrees. [Conclusion] The direct vat set lactobacillus preparation could reduce the fat accumulation in liver of obesity rats, and it might be applied to the prevention against fatty liver in the future.