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首页> 外文期刊>Journal of the American Society of Brewing Chemists >Application of Ribotyping and rDNA Internal Space Analysis (RISA) for Assessment of Microflora in Brewery Environments
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Application of Ribotyping and rDNA Internal Space Analysis (RISA) for Assessment of Microflora in Brewery Environments

机译:核糖体分型和rDNA内部空间分析(RISA)在啤酒环境中评估菌群的应用

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摘要

The applications of ribotyping and rDNA internal space analysis (RISA) were evaluated for assessment of microflora in brewery environments. For this purpose, approximately 2,000 strains isolated in our breweries were analyzed by ribotyping, and the database was constructed using all the data acquired. The strains that displayed unidentifiable ri-bopatterns were presumptively identified by 16S rDNA analysis, and novel ribopatterns were registered in our database as Custom ID patterns. As a result, it was demonstrated that ribotyping is capable of discriminating the brewery isolates on the strain level and well suited to the detailed analysis of microflora in brewery environments. We have also successfully accumulated an enormous database of the strains isolated from the brewery environments. We further evaluated RISA as an inexpensive and rapid alternative to ribotyping. As a consequence, RISA was shown to distinguish strains broadly on the species level, which is sufficient for the practical assessment of brewery isolates. Because this technique has an advantage in terms of cost and time required for analysis, RISA was also considered to complement ribotyping as a prescreening method. On the basis of these results, we have developed a reliable system for assessing the microflora in brewery environments by combining ribotyping and RISA.
机译:评估了核糖分型法和rDNA内部空间分析(RISA)的应用,以评估啤酒厂环境中的微生物区系。为此,通过核糖分型法分析了在我们啤酒厂中分离出的大约2,000个菌株,并使用所有获得的数据构建了数据库。通过16S rDNA分析推测显示了无法识别的核糖核酸模式的菌株,并且在我们的数据库中将新的核糖模式注册为Custom ID模式。结果表明,核糖分型法能够在菌株水平上区分啤酒分离株,非常适合啤酒厂环境中微生物的详细分析。我们还成功地积累了从啤酒厂环境中分离出的菌株的庞大数据库。我们进一步评估了RISA是核糖分型法的一种廉价且快速的替代方法。结果,RISA被证明可以在物种水平上广泛地区分菌株,这足以对啤酒分离株进行实际评估。由于该技术在分析所需的成本和时间方面具有优势,因此RISA还被认为可以补充核糖分型作为一种预筛选方法。基于这些结果,我们开发了一种可靠的系统,通过结合核型和RISA评估啤酒厂环境中的微生物区系。

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