Crown gall tissue culture lines of Lithospermum erythrorhizon were established by transformation of sterile seedlings with Agrobacterium tumefaciens C58. Nopaline was inspected in the crown gall and showed that the transformation was successfull. L6 line, can secret red naphthoquinone pigments that are shikonin derivatives on phytohormone free 6,7-V medium, was selected from other lines on different basic phytohormone free media(MS, 6,7-V, WP, B5).The content of shikonin in the crown gall was 1 18%(dry tissue).The study showed that crown gall tissue culture of L.erythrorhizon was a new method to produce shikonin.
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