method for obtaining transgenic plants which express a protein with hydrogenperoxidproducerende activity by transforamtion with agrobacterium rhizogenes

• 1. 一种用于获得无标记转基因植物的农杆菌转化载体组合物及其应用 [P] . 中国专利： CN103966257B . 2016.06.08
• 2. 一种用于获得无标记转基因植物的农杆菌转化载体组合物及其应用 [P] . 中国专利： CN103966257A . 2014-08-06
• 3. METHOD FOR OBTAINING TRANSGENIC PLANTS EXPRESSING A PROTEIN WITH ACTIVITY PRODUCING HYDROGEN PEROXIDE BY TRANSFORMATION BY AGROBACTERIUM RHIZOGENES [P] . CA2346153C . 2011-12-20

  机译： 通过根癌农杆菌转化获得具有活性的生产过氧化氢的转基因植物的方法

• 4. METHOD FOR OBTAINING TRANSGENIC PLANTS EXPRESSING A PROTEIN WITH ACTIVITY PRODUCING HYDROGEN PEROXIDE BY TRANSFORMATION BY AGROBACTERIUM RHIZOGENES [P] . 欧洲知识产权局专利： EP1121451B1 . 2006-02-01

  机译： 通过根癌农杆菌转化获得具有活性的生产过氧化氢的转基因植物的方法

• 5. Method for obtaining transgenic plants expressing a protein with activity producing hydrogen peroxide by transformation by agrobacterium rhizogenes [P] . 美国专利： US7094952B1 . 2006-08-22

  机译： 通过发根农杆菌转化获得表达具有活性产生过氧化氢的蛋白质的转基因植物的方法

• 1. A Simplified Seed Transformation Method for Obtaining Transgenic Brassica napus Plants [J] . SONG Li, ZHAO De-gang, WU Yong-jun, 农业科学学报（英文版） . 2009,第006期
• 2. Expression activity of the CpTI gene in transgenic rice plants [J] . LIUWuge, XUEQingzhong 中国水稻研究通报：英文版 . 2001,第003期
• 3. Regeneration of plants from callus tissues of hairy roots induced by Agrobacterium rhizogenes on Alhagi pseudoalhagi [J] . 细胞研究（英文版） . 2001,第004期
• 4. Dexamethasone-Inducible Green Fluorescent Protein Gene Expression in Transgenic Plant Cells [J] . Wei Tang, Hilary Collver, Katherine Kinken 基因组蛋白质组与生物信息学报（英文版） . 2004,第001期
• 5. New method for obtaining transgenic papaya plants by Agrobacterium-mediated transformation of somatic embryos [C] . ZHENTU YING, XIA YU, MICHAEL J. DAVIS Annual Meeting of the Florida State Horticultural Society . 2000
• 6. Agrobacterium tumefaciens Production to Enable the Large-Scale Transient Expression of Recombinant Proteins in Plants. [D] . Leth, Ingrid. 2015
• 7. Hormonal Characterization of Transgenic Tobacco Plants Expressing the rolC Gene of Agrobacterium rhizogenes TL-DNA. [O] . O. Nilsson, T. Moritz, N. Imbault, 1993
• 8. Hormonal Characterization of Transgenic Tobacco Plants Expressing the rolC Gene of Agrobacterium rhizogenes TL-DNA. [O] . Nilsson, O., Moritz, T., Imbault, N., 1993

• 1. BACKGROUND： Vibrio vulnifi cus inside the body could activate the NF-!B signaling pathwayand initiate the inflammatory cascade. The lung is one of the earliest organs affected by sepsisassociated with acute lung injury. High mobility group protein B1 （HMGB1） is an important late-actingpro-infl ammatory cytokine involving in the pathophysiology of sepsis. It is also involved in the injuryprocess in the lung, liver and intestine. There has been no report on the involvement of HMGB1 inVibrio vulnifi cus sepsis-induced lung injury.METHODS： Sixty rats were randomly divided into a normal control group （group A, n=10） anda Vibrio vulnificus sepsis group （group B, n=50）. Sepsis was induced in the rats by subcutaneousinjection of Vibrio vulnificus （concentration 6×108 cfu/mL, volume 0.1 mL/100g）） into the left lowerlimbs. The rats in group B were sacrifi ced separately 1, 6, 12, 24, and 48 hours after the infection.Their lungs were stored as specimens, lung water content was measured, and lung pathology wasobserved under a light microscope. The expressions of the HMGB1 gene and protein in the lungswere detected by RT-PCR and Western blot. Data were analyzed with one-way analysis of variance（ANOVA） and the LSD method for pair-wise comparison between the two groups. P〈0.05 wasconsidered statistically signifi cant.RESULTS： Compared to group A （0.652±0.177）, HMGB1 mRNA expression in the lungs ofgroup B was signifi cantly higher at 0 hour （1.161±0.358, P=0.013）, 24 hours （1.679±0.235, P=0.000）,and 48 hours （1.258±0.274, P=0.004） （P〈0.05）, and peaked at 24 hours. Compared to group A（0.594±0.190）, HMGB1 protein expression at 6 hours （1.408±0.567, P=0.026） after infection wassignificantly increased （P〈0. 05）, and peaked at 24 hours （2.415±1.064, P=0.000） after infection.Compared to group A （0.699±0.054）, lung water content was significantly increased at 6 hours（0.759±0.030, P=0.001）,12 hours （0.767±0.023, P=0.000）, 24 hours （0.771±0.043, P=0.000） and 48hours （0.789±0.137, P=0.000） after infection （P〈0.05）. Compared to group A, pathological changesat 12 hours in group B indicate marked pulmonary vascular congestion, interstitial edema andinfl ammatory infi ltration. Alveolar cavity collapse and boundaries of the alveolar septum could not beclearly identifi ed.CONCLUSION： Vibrio vulnifi cus sepsis can lead to injury in rat lungs, and increased HMGB1expression in lung tissue may be one of the mechanisms for injury from Vibrio vulnifi cus sepsis. [J] . Qiao-meng Qiu ,Zhong-wang Li ,Lu-ming Tang . 世界急诊医学杂志(英文) . 2011,第4期
• 2. BmK ANEPⅢ植物表达载体构建及根癌农杆菌转化条件考察 [C] . 黄婷婷 ,来琳琳 ,王轶 . 2008中国药学会学术年会暨第八届中国药师周 . 2008
• 3. 根癌农杆菌转化杜仲的初步研究 [A] . 任红媛 . 2007