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首页> 外文期刊>Journal of the American Society of Brewing Chemists >Yeast Viability by Fluorescent Staining
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Yeast Viability by Fluorescent Staining

机译：荧光染色的酵母活力

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1. Viability assessments of nonstressed cells using the fluorescent stains oxonol, berberine, and MgANS (magnesium salt of 8-anilino-1-naphthalene-sulfonic acid) were not significantly different from slide count, the current ASBC-recommended method. 2. Analysis of the viability of stressed cell populations using each fluorescent stain indicated a lesser degree of correlation to slide culture than when nonstressed cells were examined. 3. A comparison of mean variation indicated that the fluorescent stain oxonol produced results that were most similar to the 'expected' values for nonstressed and stressed cell populations. 4. When compared with the 'observed' viability (as determined by slide culture), berberine was the most accurate stain for analyzing nonstressed cell populations, while MgANS was the most efficient for stressed cultures. 5. In terms of interlaboratory reproducibility and intralaboratory repeatability, oxonol was statistically the best method overall. However, when compared with slide culture, MgANS produced the most reproducible and repeatable results for the analysis of stressed cells.
机译：1.使用荧光染色剂oxonol，小ber碱和MgANS（8-苯胺基-1-萘磺酸的镁盐）对未应激细胞的生存力评估与载玻片计数（当前的ASBC推荐方法）没有显着差异。 2.使用每种荧光染色剂对应激细胞群的生存力进行分析表明，与检查无应激细胞时相比，与玻片培养的相关性程度较低。 3.平均变化的比较表明，荧光染色的氧杂酚产生的结果与非应激和应激细胞群体的“预期”值最相似。 4.与“观察到的”生存力（由玻片培养确定）相比，小ber碱是分析非应激细胞群体最准确的染色剂，而MgANS对于应激培养最有效。 5.就实验室间的可重复性和实验室内的可重复性而言，从统计学上来说，oxonol是最好的方法。但是，与载玻片培养相比，MgANS产生的重现性和可重复性最高，可用于分析应激细胞。

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