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首页> 外文期刊>Journal of the American Society of Brewing Chemists >Microbial Attachment and Biofilm Formation in Brewery Bottling Plants
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Microbial Attachment and Biofilm Formation in Brewery Bottling Plants

机译：啤酒装瓶厂的微生物附着和生物膜形成

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摘要

Microbial attachment and biofilm formation in brewery bottling plants was studied by mounting sterile, stainless steel coupons onto critical sites of the fillers in three breweries. Microbiological samples were taken from the coupons to reveal the primary colonizers in biofilm formation. The bacteria were identified using ribotyping, carbohydrate fermentation tests, and partial 16S rDNA sequencing. The effect of sugars and sweeteners on the attachment of primary colonizers to stainless steel was analyzed with epifluorescence microscopy. The biofilm formation rate was studied for eight weeks by successively dislodging the test coupons from each sampling site and examining them with epifluorescence microscopy. The results showed that primary colonizers accumulated on new stainless steel surfaces within hours after the start of production. Regular daily cleaning reduced the number of microorganisms on the surfaces only momentarily. Canning machines were markedly less prone to accumulation of microorganisms than bottling machines. Gram-negative bacteria, yeasts, and molds were the first to colonize the surfaces. Attachment of primary colonizers to stainless steel was increased by sugars and sweeteners. Horizontal surfaces were prone to microbial accumulation and should be avoided in constructions as much as possible. Furthermore, biofilm formation occurred on certain surfaces despite daily cleaning and disinfection.
机译：通过在三个啤酒厂的灌装机关键部位安装无菌不锈钢试样，研究了啤酒灌装厂中微生物的附着和生物膜的形成。从试样中提取微生物样品以揭示生物膜形成中的主要定居者。使用核糖分型法，碳水化合物发酵测试和部分16S rDNA测序鉴定细菌。用落射荧光显微镜分析了糖和甜味剂对主要定植剂附着在不锈钢上的影响。通过连续地从每个采样位置移出测试试样并用落射荧光显微镜检查它们，研究了八周的生物膜形成速率。结果表明，主要的定殖器在生产开始后的几个小时内就堆积在新的不锈钢表面上。每天进行定期清洁只能暂时减少表面上的微生物数量。与装瓶机相比，装罐机明显不易产生微生物。革兰氏阴性细菌，酵母菌和霉菌最先在表面定殖。糖和甜味剂增加了主要定植器与不锈钢的连接。水平表面容易产生微生物积聚，应尽可能避免在结构中使用。此外，尽管每天进行清洁和消毒，但仍会在某些表面上形成生物膜。

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