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Evaluation study on C57BL/6J DIO model mice

机译：C57BL / 6J DIO模型小鼠的评估研究

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Objective: The C57BL/6J mouse as a model of Diet-Induced obesity (DIO) exhibits the biological features of obesity and mild hyperglycemia. To evaluate this model, we measured gene expression levels associated with glucose and lipid metabolism. Design: 6-week-old male mice were fed ad libitum a high-fat diet (D12492; Research Diets, Inc.), or a normal diet (D12450B; Research Diets, Inc.) for 12 weeks. Blood biochemistry and gene expression level in epididymal white adipose tissue and liver were analyzed at 18 weeks of age. Result and discussion: Lep and Ucp2 expression levels were significantly increased in white adipose tissue of high-fat diet group in comparison with normal diet group. In contrast, Adipoq, Hsdllbl and Pparg expression levels were significantly decreased in white adipose tissue of high-fat diet group. We found that the increased expression of Lep was consistent with increased serum leptin value. And the decreased expression of Adipoq was consistent with decreased serum adiponectin value. In addition, the decreased expression of Hsdllbl and Pparg may be related to development of insulin resistance. In the liver, expression of genes associated with cholesterol metabolism(Hmgcr) and lipogenic enzyme(Fasn) was significantly decreased. The decreased expression of Hmgcr may be related to increased serum TCHO value. And expression level of Fasn was significantly decreased, but serum NEFA and TG values were not different between high-fat diet group and normal diet group. Thus, serum NEFA and TG values were hardly altered, but expression level of various genes required for fatty acid and triglyceride synthesis may be altered in C57BL/6J DIO model mice.
机译：目的：C57BL / 6J小鼠作为饮食诱导型肥胖（DIO）的模型，具有肥胖和轻度高血糖症的生物学特征。为了评估该模型，我们测量了与葡萄糖和脂质代谢相关的基因表达水平。设计：给6周大的雄性小鼠随意喂养高脂饮食（D12492; Research Diets，Inc.）或正常饮食（D12450B; Research Diets，Inc.），持续12周。分析了18周龄附睾白色脂肪组织和肝脏的血液生化和基因表达水平。结果与讨论：与正常饮食组相比，高脂饮食组白色脂肪组织中Lep和Ucp2表达水平显着升高。相反，高脂饮食组的白色脂肪组织中的Adipoq，Hsdllbl和Pparg表达水平显着降低。我们发现，Lep表达的增加与血清瘦素值的增加是一致的。并且Adipoq表达的降低与血清脂联素值的降低相一致。另外，Hsdllb1和Pparg的表达降低可能与胰岛素抵抗的发展有关。在肝脏中，与胆固醇代谢（Hmgcr）和脂肪形成酶（Fasn）相关的基因的表达显着降低。 Hmgcr表达降低可能与血清TCHO值升高有关。高脂饮食组和正常饮食组的Fasn表达水平明显降低，但血清NEFA和TG值无差异。因此，血清NEFA和TG值几乎没有改变，但是脂肪酸和甘油三酸酯合成所需的各种基因的表达水平在C57BL / 6J DIO模型小鼠中可能会改变。

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