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首页> 外文期刊>Bulletin of the National Research Centre >Molecular Characterization of Wheat Triticum aestivum Cultivars Using Isoenzyme Analysis and RAPD Fingerprint
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Molecular Characterization of Wheat Triticum aestivum Cultivars Using Isoenzyme Analysis and RAPD Fingerprint

机译：同工酶分析和RAPD指纹图谱分析小麦小麦品种的分子特征

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摘要

Molecular characterization of Egyptian T. aestivum wheat cultivars including protease isoenzyme pattern and randomly amplified polymorphic DNA fingerprint (RAPD) was studied. Protease isoenzyme patterns indicated that the enzyme could exist as fast, intermediate or slow migrating forms. The numbers and relative mobilities of protease bands of these cultivars were different and ranged between 2 and 5 bands. The degree of similarity between the wheat cultivars was determined and the results obtained indicated that Baneswif 3 and Sakha 8 have 100% similarity and could not be differentiated by the protease isoenzyme patterns. DNA fingerprints of wheat cultivars were studied using 10 random primers, of which only one primer P-262 gave visible and reproducible polymorphic bands. RAPD analysis and dendrogram indicated the genetic diversity and intervarietal relationships between the cultivars and also can be used for cultivar identification. On this view, RAPD analysis can differentiate between the cultivars that can not be differentiated by protease isoenzymes such as Baneswif 3 and Sakha 8. This study constitutes a contribution to the identification of molecular markers for genetic diversity and intervarietal relationships among Egyptian wheat cultivars.
机译：研究了埃及普通小麦小麦品种的分子特性，包括蛋白酶同工酶模式和随机扩增的多态性DNA指纹图谱（RAPD）。蛋白酶同工酶模式表明该酶可以快速，中间或缓慢迁移形式存在。这些品种的蛋白酶带的数目和相对迁移率是不同的，范围在2至5个带之间。确定了两个小麦品种之间的相似度，所得结果表明，Baneswif 3和Sakha 8具有100％的相似性，不能通过蛋白酶同工酶模式区分。使用10个随机引物研究了小麦品种的DNA指纹，其中只有一个引物P-262具有可见的和可复制的多态性条带。 RAPD分析和树状图显示了品种之间的遗传多样性和品种间关系，也可用于品种鉴定。根据这种观点，RAPD分析可以区分不能被蛋白酶同功酶（例如Baneswif 3和Sakha 8）区分的品种。这项研究为鉴定埃及小麦品种之间遗传多样性和品种间关系的分子标记做出了贡献。

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