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首页> 外文期刊>Bulletin of the National Research Centre >Effect of Abscisic Acid on Some Salt Stressed Maize Inbreds
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Effect of Abscisic Acid on Some Salt Stressed Maize Inbreds

机译：脱落酸对部分盐胁迫玉米自交系的影响

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From 20 maize inbred lines, four were chosen as salt-tolerant genotypes (L123a, O251b, L113a and Rg25) and four as salt-sensitive genotypes (Rg5, G227b, Rgl and G13). These eight maize inbreds were treated with 10~(-5) M abscisic acid (ABA) under 5000 and 7000 ppm NaCl conditions. The effect of exogenous ABA on salt tolerance was tested by estimating some yield-related traits (plant height, total root length, shoot fresh weight, shoot dry weight and root dry weight). The two most salt-tolerant (L123a and G251b) inbreds and the two most salt-sensitive (Rgl and G13) were selected for detecting some biochemical and molecular genetic markers associated with salt tolerance. SDS-PAGE banding pattern showed differential responses with respect to salt tolerance and ABA treatment, while esterase and peroxidase isozyme profiles were weakly or negatively correlated with salt tolerance and ABA treatment. RAPD-PCR analysis showed that four random 10-mer primers, out of six, succeeded in differentiating salt-tolerant (L123a and G251b) from salt-sensitive (Rgl and G13) inbreds. On the other hand, some specific markers for maize inbreds across RAPD-PCR analysis were determined. Twenty-seven of 57 RAPD markers were found to be inbred-specific. Moreover, phylogenetic relationships between these inbreds were studied on the bases of RAPD-PCR patterns. Dendrogram analysis showed that, the inbreds were divided into two main groups and the most dissimilar one was the most salt-sensitive inbred G13.
机译：从20个玉米自交系中选择了四个作为耐盐基因型（L123a，O251b，L113a和Rg25），另外四个选择为盐敏感性基因型（Rg5，G227b，Rgl和G13）。这八个玉米自交系分别在5000和7000 ppm NaCl条件下用10〜（-5）M脱落酸（ABA）处理。通过估计一些与产量相关的性状（植物高度，总根长，枝鲜重，枝干重和根干重），测试了外源ABA对耐盐性的影响。选择两种最耐盐的近交（L123a和G251b）和两个最耐盐的近交（Rgl和G13）来检测一些与耐盐性相关的生化和分子遗传标记。 SDS-PAGE谱带模式显示出对盐耐受性和ABA处理的差异响应，而酯酶和过氧化物酶同工酶谱与盐耐受性和ABA处理呈弱或负相关。 RAPD-PCR分析表明，六种引物中的四种随机10-mer引物成功地将耐盐性（L123a和G251b）与对盐敏感的近亲（Rgl和G13）自交系区分。另一方面，通过RAPD-PCR分析确定了一些玉米自交系的特异性标记。发现57个RAPD标记中有27个是近交特异性的。此外，基于RAPD-PCR模式研究了这些近交系之间的系统发育关系。树状图分析表明，自交系分为两大类，最不相似的一类是对盐敏感度最高的自交系G13。

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