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首页> 外文期刊>Bulletin of the National Research Centre >Induction of Genetic Variations in Marine Streptomyces spp. Strain AH1 Towards Higher Amylase Activity
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Induction of Genetic Variations in Marine Streptomyces spp. Strain AH1 Towards Higher Amylase Activity

机译：在海洋链霉菌属物种中的遗传变异的诱导。 AH1菌株具有更高的淀粉酶活性

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摘要

Marine microorganisms have become an important field of study in the search and improvement of their microbial products. A random mutagenesis approach was utilized to isolate supersecreting mutants from the marine Streptomyces spp. AH1. A total of 157 isolates were selected following mutagenic treatment with either UV light or NTG. Out of these 58 were selected as morphological variants. Eight oversecreting mutants were isolated which produced at least 50 % and reached to 205 % more α-amylase activity than the original culture. The use of UV-irradiation was the most useful and positively effective, while NTG treatment was less effective in producing hyperamylase activity isolates. Three overyielding isolates (A32, D7 and E7) were selected, on the basis of the obtained results about α-amylase production and to either resistance or sensitivity to one or more of the five used antibacterial antibiotics, for protoplast fusion. Fifteen stable fusants were obtained and regenerated from two crosses (A32 X E7 and D7 X E7). Upon testing these fusants for α-amylase production, five fusants overyielded the higher parent. Out of them three fusants produced 46.6 % more α-amylase than their higher parent and at the same time represents 347.4 % more α-amylase than the original culture.
机译：海洋微生物已成为搜索和改进其微生物产品的重要研究领域。利用随机诱变方法从海洋链霉菌属物种中分离出超分泌突变体。 AH1。经紫外线或NTG诱变处理后，共选择了157个分离株。从这58种中选择形态学变异。分离出八个过度分泌的突变体，它们产生的α-淀粉酶活性比原始培养物至少高出50％，达到205％。紫外线照射是最有用和最有效的方法，而NTG处理在产生高淀粉酶活性分离株方面不太有效。基于获得的关于α-淀粉酶生产的结果以及对五种使用过的抗菌抗生素中的一种或多种对原生质体融合的抗性或敏感性的结果，选择了三种过分的分离株（A32，D7和E7）。从两个十字架（A32 X E7和D7 X E7）获得了15个稳定的融合剂并对其进行了再生。在测试这些融合剂的α-淀粉酶生成后，五种融合剂使高亲本的产量过高。在它们当中，三种融合剂比其较高亲本产生的α-淀粉酶高出46.6％，同时比原始培养物产生的α-淀粉酶高347.4％。

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