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首页> 美国卫生研究院文献>IEEE Journal of Translational Engineering in Health and Medicine >Precise Dispensing Technology Using Electroformed Tubes for Micro-Volume Blood Diagnosis
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Precise Dispensing Technology Using Electroformed Tubes for Micro-Volume Blood Diagnosis

机译：使用电铸管进行精确分配技术进行小批量血液诊断

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Precise dispensing of droplets is a crucial step for acquiring reliable blood diagnostic results. When the source sample volume is limited, the need for precise dispensing of submicroliter and nanoliter quantities is especially important. In this paper, we developed a positive-displacement high-precision dispensing technique using a nickel electroformed tube with an inner diameter accuracy of or less. When dispensing variation of 100 nL was evaluated by using a photometric method, the most stable coefficients of variation (CV) were observed for a tube thickness of with hydrophobic treatment, where the average CV value was 1.3%, i.e., 1.3 nL. Furthermore, the glucose concentration of 100- and 200-nL animal-based control serum was colorimetrically measured using enzymatic reactions without drying and mixing reagents. The CV value was approximately 6.36% at 100 nL and 3.23% at 200 nL, suggesting that several biochemical panels can be precisely measured even from less than one drop of blood. This positive-displacement dispenser ensures zero contamination and almost-zero dead volume, thus it would be useful for multi-panel clinical blood testing.
机译：液滴的精确分配是获得可靠的血液诊断结果的关键步骤。当源样品的体积受到限制时，精确分配亚微升和纳升量的需求就变得尤为重要。在本文中，我们开发了一种使用内径精度为或更小的镍电铸管的正排量高精度点胶技术。当使用光度法评估分配的变化为100 nL时，对于经疏水处理的管厚度，观察到最稳定的变化系数（CV），其中平均CV值为1.3％，即1.3 nL。此外，使用酶促反应无需干燥和混合试剂即可比色测定100和200 nL基于动物的对照血清的葡萄糖浓度。 100 nL时的CV值约为6.36％，200 nL时的CV值约为3.23％，这表明即使从不到一滴血中也可以精确测量出几个生化指标。这种容积式分配器可确保零污染和几乎零死体积，因此对于多面板临床血液测试将非常有用。

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