目的研究KLF6基因M1、M2、M4、D10S1716位点的微卫星不稳定性（MSI）和杂合性缺失（LOH）与原发性肝癌进展的关系，为揭示抑癌基因作用机制和肿瘤发生、发展机制提供实验依据。方法从肝癌组织标本中抽提DNA，应用PCR-单链构象多态性（PCR-SSCP）法进行KLF6基因遗传不稳定性的研究。结果肝癌KLF6基因微卫星位点LOH的检出率为35.71%（10/28），与肝癌分化程度、有无包膜无相关性（均P＞0.05），但与临床TNM分期密切相关（P＜0.01），LOH在肝癌Ⅲ～Ⅳ期检出率为（75.00%，6/8）明显高于Ⅰ～Ⅱ期（20.00%，4/20）。MSI检出率为17.86%（5/28），MSI与肝癌分化程度、淋巴转移、临床TNM分期和有无包膜均无相关性（均P＞0.05）。结论 KLF6基因的遗传不稳定性可能是原发性肝癌发生、发展的一个重要机制，KLF6基因LOH的发生率与临床TNM分期正相关。LOH在KLF6杂合性缺失的过程中起了重要作用，可作为肝癌恶化及进展的一个指标，MSI则可能影响肿瘤的预后。% Objective To investigate genetic instability of KLF6 gene in hepatocel ular carcinoma (HCC). Methods DNA was extracted from fresh tissue samples of 28 patients with hepatocel ular carcinoma. Microsatel ite instability (MSI) and loss of hererozygosity (LOH) of locus KLF6M1, KLF6 M2, KLF6 M4 and D10S1716 in KLF6 gene were examined by PCR-SSCP method. Results The detection rate of LOH in KLF6 gene of HCC was 35.71%(10/28) , which was correlated with TNM stages (P<0.01), but not correlated with the differential degrees and tumor with or without capsule (P>0.05). The detection rate of LOH in stageⅢ +Ⅳtumors was 75.00% (6/8), which was significantly higher than that in tumors of stageⅠ+Ⅱ (20.00%, 4/20). The detection rate of MSI of KLF6 gene was 17.86%(5/28), which was not correlated with differential degrees, lymph node metastasis, TNM stages and with or without capsule (P>0.05). Conclusion The genetic instability of KLF6 gene may be associated with carcino-genesis and development of HCC.
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