目的探讨肿瘤干细胞标志CD166在原发结肠癌组织中的表达及其临床生物学意义。方法收集57例患者的原位结肠癌组织，应用免疫组织化学检测其CD166表达。应用流式细胞分选（FACS）技术，以CD166/CD133标志分选结肠癌细胞株SW620，通过NOD/SCID小鼠脾脏移植，观察肝转移情况。结果 CD166可表达于结肠癌细胞的细胞质和细胞膜，其中细胞质阳性率98.2%（56/57），细胞膜阳性率42.1%（24/57）。膜CD166阳性表达与局部浸润（P=0.010）、pTNM分期（P=0.035）、远处转移（P=0.013）、年龄（P=0.026）相关；而与性别（P=0.426）、淋巴结转移（P=0.104）、肿瘤分化（P=0.171）无相关性。Log-rank生存分析显示：结肠癌细胞膜CD166阳性表达与患者生存期（P=0.014）呈负相关。动物实验的初步结果显示：脾包膜接种CD166阳性的SW620细胞可形成肝转移灶。结论 CD166阳性表达与肿瘤局部进展和远处转移有关，是判断预后不良的标志，CD166阳性的肿瘤细胞可能具有肝转移潜能。% Objective To investigate the expression and biological significance of CD166 in primary colon cancer. Meth-ods The expression of CD166 was detected by immunohistochemistry in tissue specimens from 57 patients with colon cancer of various pTNM stages. Human colon cancer SW620 cel s were screened for positive expression of CD166 and CD133. The CD166 and CD133-positive SW620 cel s were inoculated in spleen of NOD/SCID mice;then the ability of liver metastases of transplanted SW620 tumors was evaluated. Results The CD166 was positively expressed in cytoplasm and membrane of colon cancer cel s, being 98.2%(56/57) in cytoplasm and 42.1%(24/57) in membrane of cancer cel s. CD166 membrane expression was significantly associated with older age(P=0.026), tumor stage (P=0.035), and distance metastasis(P=0.013), not with gender(P=0.426), lymph node metastasis (P=0.104) and tumor differentiation (P=0.171). Log-rank survival analysis revealed that CD166 membrane ex-pression was negatively correlated with the survival of patient (P=0.014). The results of the animal experiments demonstrated metastatic growth in the liver after splenic capsule injection CD166+ SW620 cel s. Conclusion The expression of membrane CD166 is associated with poor clinical outcome and short survival in the patient with colon cancer. CD166+colon cancer cel s may have a potential of liver metastasis.
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