目的观察人参二醇对鱼藤酮、MPP+诱导的大鼠嗜铬细胞瘤损伤的影响。方法以大鼠嗜铬细胞瘤PC12细胞为研究对象，以鱼藤酮（0.3、1、3、10、30μmol/L）或MPP+（0.1、0.3、1、3mmol/L）诱导细胞损伤。设阴性对照组、人参二醇对照组（10、25、50、75、100mg/L）、鱼藤酮（3μmol/L，24h）或MPP+（1mmol/L，48h）组，人参二醇（10、25、50、75、100mg/L）联合鱼藤酮（3滋mol/L）或MPP+（1mmol/L）组。采用MTT法检测细胞增殖活性；PI和Hoechst 33342染色检测细胞坏死和凋亡；并以免疫组织化学测定细胞酪氨酸羟化酶（TH）的表达。结果人参二醇（10、25、50、75、100mg/L）本身不会抑制PC12细胞增殖，其中50、75mg/L人参二醇还可促进细胞增殖；各浓度人参二醇对鱼藤酮诱导的细胞损伤均不具有保护作用；在MPP+处理诱导细胞损伤后，50、75mg/L人参二醇可提高细胞增殖活性，但人参二醇对细胞凋亡和坏死及TH的表达无影响。结论人参二醇（50、75mg/L）对MPP+诱导的PC12细胞损伤有保护作用，其作用机制与促进细胞增殖有关；人参二醇对鱼藤酮诱导的细胞损伤无明显保护作用。% Objective To evaluate the effects of panoxadiol on rat pheochromocytoma PC12 cel injury induced by rotenone or MPP+. Methods PC12 cel injury was induced by rotenone (0.3, 1, 3, 10, 30μmol/L,24h) or MPP+ (0.1, 0.3, 1, 3 mmol/L, 48h). Panoxadiol (10, 25, 50, 75,100 mg/L) was used to treat rotenone or MPP+induced PC12 cel s. Cel proliferation was assessed by MTT method, cel necrosis and apoptosis was detected by flow cytometry with PI (propidium iodide) and Hoechst 33342 stain, respectively. The expression of tyrosine hydroxylase was measuared via immunohistostaining. Results Panoxadiol (10, 25, 50, 75, 100mg/L) had no affcts on proliferation of PC12 cel s and had no protection effects on cel injury induced by rotenone. When PC 12 cel injury was induced by MPP+, panoxadiol(50,75 mg/L) promoted cel proliferation but had no effects on necrocytosis and apoptosis and the expression of tyrosine hydroxylase. Conclusion Panoxadiol (50、75 mg/L) might have pro-tection effects on PC12 cel injury induced by MPP+, which may be related to facilitation of cel proliferation;however, panoxadiol has no protection effects on cel injury induced by rotenone.
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