Objective To transfect human sodium/iodide symporter gene hNIS into colon cancer SW480 cel s and to ex-amine the hNIS mRNA and protein expression. Methods The recombinant plasmid (pcDNA3.1+-hNIS) was constructed, identi-fied and transfected into SW480 cel s with lipofectamine 2000. Reverse transcription-polymerase chain reaction (RT-PCR) and Western blot were applied to detect the hNIS mRNA and protein expression. Results Enzyme digestion and DNA sequencing confirmed the size and direction of the recombinant (pcDNA3.1+-hNIS) plasmid. RT-PCR and Western blot showed the expres-sion of hNIS mRNA and protein in pcDNA3.1+-hNIS-transfected SW480 cells, not in non-transfected cel s or blank plas-mid-transfected cel s. Conclusion The hNIS gene is effectively transfected into human colon cancer SW480 cel s and the NIS protein is expressed successful y.% 目的以重组人钠/碘转运体（hNIS）基因转染结肠癌SW480细胞并检测hNIS mRNA及蛋白的表达，为放射性碘治疗非甲状腺肿瘤提供新思路。方法将构建好的重组质粒（pcDNA3.1+-hNIS）进行酶切、测序鉴定并扩增、提取。SW480细胞分为重组质粒（pcDNA3.1+-hNIS）转染组、空白质粒（pcDNA3.1+）转染组、空白对照组，转染后以RT-PCR和Western blot检测各组细胞hNIS mRNA和蛋白的表达。结果酶切和测序结果显示插入的hNIS基因大小和方向均正确。RT-PCR和Western blot显示重组质粒转染组SW480细胞可见hNIS mRNA和蛋白的表达，而空白对照组和空白质粒转染组均未检测到hNIS mRNA和蛋白的表达。结论脂质体法可有效地将hNIS基因转染至SW480细胞并成功表达hNIS蛋白。
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