目的 建立高效液相测定剑叶龙血树人工诱导结血物中龙血素A、B的方法,用于筛选有效的剑叶龙血树结血诱导剂.方法 高效液相色谱法(HPLC)法,色谱柱安捷伦ZORBAX Eclipse XDB C18(4.6 mm ×250 mm,5 μm),流动相为乙腈-1％冰醋酸(40:60),流速1.0 mL/min,柱温25 ℃,检测波长为280 nm.结果 龙血素A在0.0216～0.1948 μg范围内呈线性关系,回归方程为Y=-0.7836X +1.8251,r=0.9998,龙血素B在0.0201～0.1811 μg范围内呈线性关系,回归方程为Y=-0.4785X +1.0548,r=0.9997,不同诱导剂诱导所得龙血素A含量在0.013％～0.099％,龙血素B含量在0.044％～0.097％.结论 该方法快速、准确,可用于剑叶龙血树人工诱导结血物中龙血素A、B的检测,茉莉酸甲酯(50 mg/L)-苯甲酸(2 g/L)、硫酸镁(5％)-苯甲酸(2 g/L)、3吲哚丁酸(5 mg/L)-苯甲酸(2 g/L)、的诱导结血效果较好.%A determination method of Loureirin A and Loureirin B in artificially induced dragon's blood of Dracaena cochinchinensis by HPLC was established to screen effective inducer.The Loureirin A and Loureirin B were separated on Agilent ZORBAX Eclipse XDB C18(4.6 mm×250 mm,5μm). UV detection wavelength was at 280 nm. The results indicated that the Loureirin A content showed good linear in the range of 0.021 6～0.194 8 μg,regression equation: Y=-0.783 6X+1.825 1,correlation coefficient(r)=0.999 8;The Loureirin B content showed good linear in the range of 0.0201～0.1811 μg,regression equation: Y=-0.4785X+1.054 8,correlation coefficient(r)=0.999 7.The content of Loureirin A ranged from 0.013％ to 0.099％ and the content of Loureirin B ranged from 0.044％ to 0.097％.The determination method of Loureirin A and Loureirin B in artificially induced dragon's blood of Dracaena cochinchinensis is simple and repeatable, and can be used for screening the effective inducer of dragon's blood of Dracaena cochinchinensis.
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