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OPTICAL PROPERTIES OF NANOCHLOROPSIS SP. AND THEIR APPLICATION TO REMOTE ESTIMATION OF CELL MASS

机译:纳米氯化物SP的光学性质。及其在远程估计细胞质量的应用

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The absorption and scattering coefficients and reflectance spectra of ultrahigh density Nannochloropsis occulata cultures were investigated in detail in order to identify the optical properties of the cultures and devise algorithms for remote estimation of dry cell mass in ultrahigh cell density cultures. High spectral resolution measurements of apparent absorption and attenuation as well as reflectance from 400 to 900 nm were carried out in relation to the dry mass, cell count and pigment concentration in outdoor cultures. Indices calculated as (R_(NIR)-R_(red))/(R_(NIR)+R_(red)) and R_(NIR)/R_(red), in which R_(NIR) is reflectance in the range from 750 to 800 nm and R_(red) is reflectance in the range 670-680 nm, respectively, were used for remote assessment of dry cell mass. Remote estimation in the range 1 to 8 g/L was accomplished with an error of less than 0.66 g/L. A different index, i.e. (R_(NIR)-R_(red)) was employed for estimation of cell-chlorophyll concentration. This is the first report of in-vivo specific absorption coefficient of chlorophyll-a and specific scattering coefficient per dry algal mass of Nannochloropsis sp., providing a basis for remote monitoring of dense phytoplankton masses.
机译:详细研究了超高密度Nannchlopsis Occulata培养物的吸收和散射系数和反射光谱,以鉴定培养物的光学性质和用于远程细胞密度培养物中的干细胞块的远程估计的光学性质。在室外培养物中的干料,细胞计数和颜料浓度,对表观吸收和衰减以及400至900nm的反射率的高光谱分辨率测量。计算为(r_(nir)-r_(红色))/(r_(nir)+ r_(红色))和r_(nir)/ r_(红色),其中r_(nir)是从750的范围内反射率对于800nm和r_(红色)是670-680nm的反射率,用于远程评估干细胞质量。在1至8g / L范围内的远程估计以小于0.66g / L的误差完成。使用不同的指标,即(R_(nir)-r_(红色))用于估计细胞 - 叶绿素浓度。这是每种干燥藻类质量的Nannchloropsis SP的叶绿素-A和特异性散射系数的体内特异性吸收系数的第一个报告。,为远程监测致密植物植物质量提供依据。

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