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首页> 外文会议>International Conference on Food Science and Technology(ICFST) vol.2; 20031022-24; Wuxi(CN) >Screen and Qualitatively Detect Ingredient of Genetically Modified Soybean by Multiplex Fast-PCR
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Screen and Qualitatively Detect Ingredient of Genetically Modified Soybean by Multiplex Fast-PCR

机译：多重快速PCR筛选和定性检测转基因大豆成分

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摘要

Designing and screening suitable primers for amplifying CaMY35S gene and soybean lectin gene by multiplex PCR, which could detect if foodstuffs exist ingredients of genetically modified (GM) soybean. The time of multiplex PCR was finished within 50 minutes; Designing and screening four primers for amplifying promoter CaMV35S - petunia CTP4 gene and cp4-epsps gene respectively, which only exist in genetically modified soybean Roundup Ready. This multiplex PCR could detect if there existed ingredient of GM soybean Roundup Ready in foodstuffs within 70 minutes. Not only could screen GM soybean but also could detect GM soybean Roundup Ready by two multiplex PCR system. The methods could not only reduce the time of detect and expense, but improve efficiency of job as well.
机译：通过多重PCR设计和筛选合适的引物，用于扩增CaMY35S基因和大豆凝集素基因，可以检测食品中是否存在转基因大豆成分。多重PCR的时间在50分钟内结束；设计和筛选分别用于扩增启动子CaMV35S-矮牵牛CTP4基因和cp4-epsps基因的四种引物，这些引物仅存在于转基因大豆抗农达蛋白中。该多重PCR可以在70分钟内检测食品中是否存在转基因大豆抗农达成分。通过两个多重PCR系统不仅可以筛选转基因大豆，还可以检测转基因大豆综述。该方法不仅可以减少检测时间和费用，而且还可以提高工作效率。

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